Characterisation of Chrysoporthe cubensis and Chrysoporthe deuterocubensis, the Stem Canker Diseases of Eucalyptus spp. in a Forest Plantation in Malaysia

Commercial plantations of Eucalyptus species have been established in Malaysia, especially during the past 10 years, with aim sustaining supply wood and timber products for industrial use Malaysia. As part an assessment fungal diseases affecting four regions including Kelantan, Pahang, Sabah, Selangor, stem canker disease was discovered to be a widespread infecting This study aimed identify fungus-causing disease, test its pathogenicity Eucalyptus, determine mating type isolates from infected trees. The fungi were identified based on morphology through comparisons DNA sequence data ITS, β-tubulin 2 gene, TEF-1α gene regions. Phylogenetic analyses showed that causal agent Chrysoporthe cubensis Pahang deuterocubensis Selangor. We believe this is first report cubensis-infected Malaysia Southeast Asia, while first-reported pellita Moreover, fact mating-type MAT1-1 MAT1-2 genes pheromone ppg1, ppg2, pre1, pre2 all indicates are homothallic systems. Pathogenicity tested 3-year-old standing tree, 1-year-old seedling, detached healthy leaves, which re-isolated fulfilling Koch’s postulates. In trials, both gave rise lesions wounded Eucalyptus. Both spp. equally pathogenic urograndis should regarded as biosecurity concern Malaysia’s forest plantation industry.